Recombinant DNA technology depends on the ability to cut and merge longer pieces of DNA at a specific base sequences.
With this technology, pieces of DNA molecules can be transferred to a virus, bacteria or yeast then amplified, isolated and identified.
With this technology, pieces of DNA molecules can be transferred to a virus, bacteria or yeast then amplified, isolated and identified.
The use of this technology: gene mapping, disease diagnosis, commercial products, trans-specific genes, vaccines and drugs.
Also: raises fears epidemic and widespread ecological changes.
Recombinant DNA technology using nucleic acid biochemistry combined with gene engineering.
Also: raises fears epidemic and widespread ecological changes.
Recombinant DNA technology using nucleic acid biochemistry combined with gene engineering.
The pieces are then inserted into another DNA molecule that serves as a vector. Vector containing the DNA fragment is referred to as recombinant DNA molecule.
Vectors that carry foreign DNA fragments are then transferred into the host cell. In the body of the host vector (along with pieces of DNA inserts) can replicate and form many copies of the inserted DNA fragments TSB. Identical copy is called clones (clones).
Cells host cell then lowered recombinant DNA molecules is the child is forming a population of cells that carry the sequence clones.
Cut pieces of DNA clones can be separated from the host cells to do the process of purification and analysis.
DNA clones can be transcribed, translated mRNAnya and their gene products were isolated and studied.
Cut pieces of DNA clones can be separated from the host cells to do the process of purification and analysis.
DNA clones can be transcribed, translated mRNAnya and their gene products were isolated and studied.
Restriction Enzyme
The most important group of enzymes in recombinant technology is of a class of restriction endonucleases.
This enzyme was isolated from bacteria, and can degrade chromosomal virus that infects bacteria.
Therefore this restriction enzymes that have the privilege of being able to recognize a specific nucleotide sequence and at the same time is able to cut the sequence.
Places recognition (recognition site) the nucleotide sequence is palindromik, which can be read from the front or the rear, can be read from the 5'- to 3 'or otherwise of thread strands of DNA.
This enzyme was isolated from bacteria, and can degrade chromosomal virus that infects bacteria.
Therefore this restriction enzymes that have the privilege of being able to recognize a specific nucleotide sequence and at the same time is able to cut the sequence.
Places recognition (recognition site) the nucleotide sequence is palindromik, which can be read from the front or the rear, can be read from the 5'- to 3 'or otherwise of thread strands of DNA.
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